Tennessee River
652 river miles
Paducah, KY to Knoxville, TN
The whole Tennessee River is part of M-65. From Paducah, it runs south through Kentucky Lake to Pickwick Lake, where the Tenn-Tom begins, then east across North Alabama to Chattanooga and Knoxville.
The water itself flows the other way, from Knoxville toward Paducah.
- Nine locks
- Kentucky, Pickwick, Wilson, Wheeler, Guntersville, Nickajack, Chickamauga, Watts Bar and Fort Loudoun
- Channel
- 11-foot minimum depth maintained by TVA
- 300 feet wide
Tennessee-Tombigbee Waterway
234 miles
Pickwick Lake to Demopolis, AL
The heart of M-65. The Tenn-Tom links the Tennessee River to the Tombigbee, giving the Tennessee Valley a direct water route to the Gulf.
- Ten locks
- Each 110 by 600 feet
- 341 feet of lift in all
- Channel
- 9 feet deep in the river sections, 12 feet in the canal and Divide Cut
- 300 feet wide, 280 in the Divide Cut
Black Warrior River
168 river miles
Demopolis to the Port of Birmingham, AL
The Black Warrior carries traffic northeast from Demopolis toward Birmingham. Its 168 river miles end where the Mulberry and Locust forks meet; the Port of Birmingham sits a short way up the Locust Fork, on the same pool.
- Four locks
- Selden, Oliver, Holt and Bankhead
- Channel
- 9 feet deep
- 200 feet wide
Tombigbee River
172 river miles
Demopolis to the Mobile River
Below Demopolis, the Tennessee and Birmingham routes travel together on the Tombigbee toward the coast.
- Two locks
- Demopolis and Coffeeville
- Coffeeville is the last lock before tidewater
- Channel
- 9 feet deep
- 200 feet wide
Mobile River
45 river miles
To the Port of Mobile, AL
The Mobile River brings M-65 to the Port of Mobile. With its 50-foot ship channel, finished in October 2025, Mobile is the deepest container port on the Gulf.
- Channel
- Upper river: 9 feet deep, 200 feet wide
- Mobile Harbor: 50 feet deep
Where M-65 connects
M-65 links to two other Marine Highways and an interstate.
- M-55
- At Paducah, the Tennessee River meets the Ohio. About 45 miles downstream, at Cairo, Illinois, M-65 connects to M-55.
- M-10
- At Mobile, M-65 connects to M-10, which runs along the Gulf Coast.
- Interstate 65
- On land, the route follows I-65.